PhosphorylationSite-DetectorRAF1
ProductCode:PD-056
Peptidemicroarraydisplaying319peptidesderivedfromapeptidescan(13merswith11aaoverlap)throughRAF1(Swiss-ProtID:P04049)ofHomosapiens(Human).
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Phosphositedetection,Substrateidentification
Indication(s)/Topic(s):Kinase
DeliveryTime:10-12days
PhosphorylationSite-Detectors
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesenablingcharacterizationofenzymessuchaskinasesaswellasmethyl,glycosyl,acetyltransferases.PeptidesareimmobilizedonglassslidesviaaflexIBLelinker.Chemoselectivecouplinggeneratesmicroarraysdisplayingcovalentlyattachedpeptidesinfouridenticalsubarrays(3copiesofpeptidewithineachsubarray).Incubationcanbeperformedmanuallyorautomated.
BenefitsofJPT"sPhosphorylationSite-Detector
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourenzymepreparationisneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,lysate,kinase)
-Lowbackgroundonglasssurface
-Read-outusingfluorescenctlylabeledantibodiesorrADIoisotopicallylabeledATP
-Screenthousandsofpotentialpeptidesubstrateseconomically
PhosphorylationSite-DetectorRAF1
SelectedReferences
"Parvulin17-CatalyzedTubulinPolymerizationisRegulatedbyCalmodulininaCalcium-DependentManner"
Burgardtetal,JBiolChem.(2015)-PMID:25940090
"PhosphopeptideDetectionwithBiotin-LabeledPhos-tag"
Kinoshita-Kikutaetal.,MethodsinMolecularBiology (2016)-PMID:26584916
"PKc-thetaisaNovelsc35SplicingFactorRegulatorinResponsetoTcellActivation"
McCuaigetal.,FrontiersinImmunology -PMID: n.a.
"Phos-tagTechnologyforKinomics"
Kinoshita-Kikutaetal.,Kinomics:ApproachesandApplications(2015)-PMID:n.a.
"CharacterizationoftheHostResponsetoPichindeVirusInfectionintheSyrianGoldenHamsterbySpecies-SpecificKinomeAnalysis"
Falcinellietal.,Mol.Cell.Proteomics.(2015)-PMID:25573744
"AntiviralPotentialofERK/MAPKandPI3K/AKT/mTORSignalingModulationforMiddleEastRespiratorySyndromeCoronavirusInfectionasIdentifiedbyTemporalKinomeAnalysis"
Kindrachuketal.,AntimicrobAgentsChemother.(2015)-PMID:25487801
ApplicationNotesforEnzymeSubstrateMicroarrays
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
"UnravelingPhosphorylationoftheSplicingFactorSC35bytheDualSignalingKinasePKCinHumanT-Cells"
Raoetal.,ApplicationNote(2016)(fulltext)
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistoneModifyingEnzymeComplex"
Wilczeketal.,ApplicationNote(2013)(fulltext)
Testimonial
"WithJPT´speptidemicroarrayplatform,high-throughputkinaseprofilingusingsmallamountsofcelllysatebecomesfeasible,providingaccesstothediscoveryandmonitoringofnovelbioMarkersinParkinson"sDisease."
JeremyNichols,Parkinsons"sInstitute,Sunnyvale,USA
PhosphorylationSite-DetectorRAF1
| Application | Phosphositedetection Substrateidentification |
| ProteinName | RAF1 |
| Organism | Homosapiens(Human) |
| Indication/Topic | Kinase |
| Numberofpeptides | 319 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededformanualincubation) |
| Specifications | Peptidescan(13merswith11aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | P04049 |
| NCBI | NP_004575 |
| Sequence | MEHIQGAWKTISNGFGFKDAVFDGSSCI SPTIVQQFGYQRRASDDGKLTDPSKTSN TIRVFLPNKQRTVVNVRNGMSLHDCLMK ALKVRGLQPECCAVFRLLHEHKGKKARL DWNTDAASLIGEELQVDFLDHVPLTTHN FARKTFLKLAFCDICQKFLLNGFRCQTC GYKFHEHCSTKVPTMCVDWSNIRQLLLF PNSTIGDSGVPALPSLTMRRMRESVSRM PVSSQHRYSTPHAFTFNTSSPSSEGSLS QRQRSTSTPNVHMVSTTLPVDSRMIEDA IRSHSESASPSALSSSPNNLSPTGWSQP KTPVPAQRERAPVSGTQEKNKIRPRGQR DSSYYWEIEASEVMLSTRIGSGSFGTVY KGKWHGDVAVKILKVVDPTPEQFQAFRN EVAVLRKTRHVNILLFMGYMTKDNLAIV TQWCEGSSLYKHLHVQETKFQMFQLIDI ARQTAQGMDYLHAKNIIHRDMKSNNIFL HEGLTVKIGDFGLATVKSRWSGSQQVEQ PTGSVLWMAPEVIRMQDNNPFSFQSDVY SYGIVLYELMTGELPYSHINNRDQIIFM VGRGYASPDLSKLYKNCPKAMKRLVADC VKKVKEERPLFPQILSSIELLQHSLPKI NRSASEPSLHRAAHTEDINACTLTTSPR LPVF |
| ProteinLength(aa) | 648 |
JPT肽技术有限公司是创新的基于肽的服务以及产品目录和试剂盒的领先提供商，也是免疫学，蛋白质组学和药物发现项目的研发合作伙伴。JPT的总部和生产基地位于德国柏林。根据DIN EN ISO 9001：2015指南，其所有生产和服务均在德国柏林进行。与合作伙伴公司JPT Peptide Technologies Inc.
JPT总部位于马萨诸塞州波士顿附近的Acton，为制药和生物技术行业的全球客户群以及大学，政府和非营利组织的研究人员提供服务。在过去的十年中，JPT开发了一系列专有技术和一系列独特的产品和服务，以支持蛋白质组学，新型疫苗或免疫疗法的所有开发阶段以及基于肽的药物发现的研究工作。
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